Within the Santa Catarina River, 87 species, 77 genera and 37 high-stratum families (trees) have been recorded, among which 18 are introduced species and the rest are native species. As for the lower stratum, that is, herbaceous, 25 families have been sighted, which belong to 53 genera and 60 species. Of the total species, 10 are introduced species. Among the various native species are the Yucca filifera, the Black Willow, the Palo Blanco, the Tronadora, the Frémont Poplar, the Palo Verde, the Yellow Agave, the American Sycamore, the Nopal, the Anacua, the Huizache, the Guamúchil , the Cruceta, the sweet Mezquite, and the Chaparro prieto. On the other hand, among the native herbaceous plants are the Roserillo, the Sunflower, the Indian Bejuco, the Tule and the Mexican Oregano.

Regarding the fauna that lives in the river, there are more than 800 species distributed in various areas, within which you can find mammals such as the opossum, the Mexican loose-tailed bat, the American beaver; reptiles of the order of the blind snake, the blue-bellied spiny lizard; birds such as the pirirí tyrant, the raven wren, the crow and the pantropical jumping spider, the giant cicada or the spotted garden spider, and many others.